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What is Dispase?

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It is a protease purified from the culture medium solution of bacillus genus strain isolated and selected from nature,and can be used as an enzyme for tissue culture.

Characteristics

  • Since the enzyme activity is hardly inhibited by serum protein, Dispase demonstrates its action in the ordinary medium including the serum.
  • The survival of cell is hardly influenced in the medium that contains serum.
  • Because of the comparatively broad range of applicable conditions, Dispase is expected to demonstrate sufficient action without a decrease in the activity and without causing cell destruction even if some discrepancy occurs in the required enzyme concentration, processing time and pH.
  • Since the action of Dispase persists for a long time under the temperature kept at 37°C, floating culture of animal cells is easily done when this enzyme is added to the medium.
  • Because it is an enzyme originated from microorganism, there is no risk of mycoplasma mixture.
  • Since Dispase shows a tendency not to disperse epithelial cells in the presence of Ca2+ while it disperses fibroblast, this enzyme may be utilized in the differentiation of cells.

Usage conditions

  • Concentration
    100 - 2,000PU/ml
  • Time
    30 minutes - several days
  • Temperature
    Room temperature - 37°C
  • pH
    6.5 - 9.0